Coding

Part:BBa_K4759050

Designed by: Shiyue Zhao   Group: iGEM23_Jiangnan-China   (2023-10-05)


GFP11-linker-OleP

Generally, the method of determining whether the redox partners was suitable required tedious steps such as the construction of plasmids, heterologous expression, construction of catalytic systems, and detection of conversion rate after catalysis. Therefore, we wanted to find a convenient way to do a quick screening. We used the fluorescent protein sfGFP and successfully constructed a sensor to detect redox partners. We divided sfGFP into N-terminal and C-terminal, and although these two parts were cut off, there was an interaction force between them. iron redox proteins were fused to the N-terminal of sfGFP-1-10 and Olep to the C-terminal of sfGFP-11, respectively

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 112
    Illegal AgeI site found at 210
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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